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Merck

PC408

Anti-Green Fluorescent Protein (26-39) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-GFP

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
(GFP and some GFP fusions)
Application:
—
Citations:
3

Key Documents

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biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

(GFP and some GFP fusions)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:2000)
Immunocytochemistry (1:200))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Immunogen

a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
Aequora victoria

Application

Immunoblotting (1:2000)

Immunocytochemistry (1:200)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yan Zhang et al.
Nature, 615(7954), 884-891 (2023-03-17)
Calcium imaging with protein-based indicators1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity and kinetics. Here
Kathryn A Tworkowski et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(16), 6103-6108 (2008-04-17)
Adenovirus E1A drives oncogenesis by targeting key regulatory pathways that are critical for cellular growth control. The interaction of E1A with p400 is essential for many E1A activities, but the downstream target of this interaction is unknown. Here, we present
Chih-Hao Lu et al.
Nature communications, 13(1), 3093-3093 (2022-06-03)
The glycocalyx is a shell of heavily glycosylated proteins and lipids distributed on the cell surface of nearly all cell types. Recently, it has been found that bulky transmembrane glycoproteins such as MUC1 can modulate membrane shape by inducing membrane

Global Trade Item Number

SKUGTIN
PC408-100UL04055977224573

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