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MABT391

Sigma-Aldrich

Anti-Myosin-10 Antibody, clone 5H4.1

clone 5H4.1, from mouse

Synonym(s):

Myosin-10, Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Non-muscle myosin heavy chain B, NMMHC-B, Non-muscle myosin heavy chain Iib, NMMHC II-b, NMMHC-IIB

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5H4.1, monoclonal

species reactivity

human, mouse

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001242941

UniProt accession no.

P35580

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYH10(4628)

General description

Myosin-10, also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Non-muscle myosin heavy chain B, NMMHC-B, Non-muscle myosin heavy chain IIb, or NMMHC-IIB, and encoded by the gene MYH10, is a myosin protein found in non-muscle cells. Often called cellular myosin, Myosin-10 appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. For instance Mysoin-10, during cell spreading, plays an important role in cytoskeleton reorganization, focal contacts and lamellipodial extension. Additionally, Myosin-10 is involved in many developmental and cellular functions including: axon guidance, retina development, cerebellar Purkinje cell layer development, exocytosis, embryonic development, mitotic cytokinesis, neuron migration, nuclear migration, regulation of cell shape, retina development, cardiac myofibril assembly, and ventricular cardiac muscle cell and adult heart development. Myosin-10 is localized to cell projections and lamellipodia. Myosin-10 is expressed in many cells and particularly in neurons in the cerebellum, spinal cord, and retina.

Immunogen

GST-tagged recombinant protein corresponding to human Myosin-10.

Application

Detect Myosin-10 using this Anti-Myosin-10 Antibody, clone 5H4.1 validated for use in western blotting.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Myosin-10 in 10 µg of HUVEC cell lysate.

Quality

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Myosin-10 in 10 µg of C2C12 cell lysate.

Target description

~230 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hiroyuki Nakajima et al.
Journal of cell science, 123(Pt 4), 555-566 (2010-01-28)
Cell-shape change in epithelial structures is fundamental to animal morphogenesis. Recent studies identified myosin-II as the major generator of driving forces for cell-shape changes during morphogenesis. Lulu (Epb41l5) is a major regulator of morphogenesis, although the downstream molecular and cellular
Marta Gai et al.
Molecular biology of the cell, 22(20), 3768-3778 (2011-08-19)
The small GTPase RhoA plays a crucial role in the different stages of cytokinesis, including contractile ring formation, cleavage furrow ingression, and midbody abscission. Citron kinase (CIT-K), a protein required for cytokinesis and conserved from insects to mammals, is currently

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