MABS466

Anti-Trpv4 Antibody, clone 1B2.6

clone 1B2.6, from mouse

• Synonym(s): Trpv4, Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Transient receptor potential protein 12, TRP12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, VRL-2, Vanilloid recep
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1B2.6, monoclonal
• Application: ICC, WB
• Citations: 8

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B2.6, monoclonal
• species reactivity: mouse, human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001170899
• UniProt accession no.: Q9HBA0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TRPV4(59341)

Description

General description

Transient receptor potential cation channel subfamily V member 4 is a multitransmembrane non-selective cation channel encoded by the TRPV4 (also known as CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VR-OAC, VRL-2, VRL2, VROAC) gene and belong to the OSM9-like transient receptor potential channel (OTRPC) subfamily within the transient receptor potential (TRP)3 superfamily of channels. Ankyrin (ANK) repeats/domains are shown to be important for functional channel expression, and ANK domain mutations are found in patients suffering from Spinal muscular atrophies (SMA, also known as hereditary motor neuropathies) and hereditary motor and sensory neuropathies (HMSN). (PMID 16293632, 20037587, 20037588).

~86-98 kDa observed. Uniprot describes 6 isoforms produced by alternative splicing ranging from ~86 to 98 kDa Uncharacterized band(s) may appear in some lysates.

Immunogen

GST-tagged recombinant protein corresponding to human Trpv4.

Application

Anti-Trpv4 Antibody, clone 1B2.6 is an antibody against Trpv4 for use in Western Blotting and Immunocytochemistry.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Trpv4 in NIH/3T3 cells.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Biochem/physiol Actions

This antibody is expected to react to all isoforms.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in SK-MEL-28 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Trpv4 in 10 µg of SK-MEL-28 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable