MABS277

Anti-monoglycylated Tubulin Antibody, clone TAP 952

clone TAP 952, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: TAP 952, monoclonal
• Species reactivity: human, sheep, sea urchin, porcine, mouse, Drosophila, snail
• Application: DB, IF, WB
• Citations: 12

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TAP 952, monoclonal
• species reactivity: human, sheep, sea urchin, porcine, mouse, Drosophila, snail
• technique(s): dot blot: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1κ
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TUBA1A(7846)

Description

General description

Several post-translational modifications of tubulin have been identified in eukaryotic cells. Glycylation is a poly-modification which occurs as lateral branching of glycine chains of variable length identified in axonemal tubulin of Paramecium cilia. This modification has been detected on tubulin and/or cilia/flagella of many unicellular and pluricellular organisms. The differential distribution of distinct polyglycylated tubulin isoforms between cytoplasmic and axonemal compartments in Paramecium indicates that the polyglycylation level of tubulin is highly regulated at the cell level. In Paramecium, the TAP 952 antibody is reactive on cytoplasmic and axonemal tubulin. In metazoan cells and/or cell lines, it is specific for tubulin of motile cilia and primary cilia, and thus useful for motile cilia and primary cilia detection.

~50 kDa observed

Immunogen

Electroeluted Paramecium axonemal tubulin

Epitope: Amino acids 427-449 of monoglycylated alpha-tubulin and 427-442 of monoglycylated beta-tubulin (Bré et al., 1998, Mol. Biol. Cell 9, 2655-2665)

Application

Detect Tubulin using this mouse monoclonal antibody, Anti-monoglycylated Tubulin Antibody, clone TAP 952 validated for use in western blotting, Immunofluorescence & Dot Blot.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in mouse spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Dot Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in synthetic monoglycylated tubulin peptides (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Western Blot Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in a panel of total protein extracts from select species (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Immunoflourescence Analysis: A representative lot from an independent laboratory detected monoglycylated Tubulin in lemur, human, and sea urchin spermatozoa (Bre, M. H., et al. (1996). J Cell Sci. (Pt 4):727-738.).

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Biochem/physiol Actions

This antibody recognizes the C-terminus of monoglycylated alpha and beta-tubulins.
Can be used as a marker for motile cilia.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting on Paramecium total cytoskeletal extract.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected monoglycylated Tubulin in 10 µg of Paramecium total cytoskeletal proteins.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable