MABN2708
Anti-Islet-1/2 homeobox Antibody, clone 39.4D5
Synonym(s):
ISL LIM homeobox 1, ISL LIM homeobox 2, Insulin gene enhancer protein ISL-1, Insulin gene enhancer protein ISL-2, Islet-1, Islet-2
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
39.4D5, monoclonal
mol wt
calculated mol wt 39.04 kDa
observed mol wt ~43 kDa
purified by
using protein G
species reactivity
human, rat, chicken, mouse, zebrafish, frog
packaging
antibody small pack of 100
technique(s)
ChIP: suitable
electron microscopy: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
rat ... , Isl2(64444)
Specificity
Clone 39.4D5 is a mouse monoclonal antibody that specifically detects Islet-1/2 homeobox. It targets an epitope within 172 amino acids from the C-terminal region.
Immunogen
C-terminal recombinant protein of rat Islet-1.
Application
Quality Control Testing
Evaluated by Western Blotting in rat hypothalamus tissue lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected Islet-1/2 homeobox in rat hypothalamus tissue lysate.
Tested Applications
Flow Cytometry Analysis: A representative lot detected Islet-1 and 2 homeobox in Flow Cytometry applications (Lei, I., et al. (2020). Front Cell Dev Biol. 7: 358).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Islet-1 and 2 homeobox in Chromatin Immunoprecipitation applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Ma, Q., et al. (2020). Cell Rep. 31(3): 107532).
Immunocytochemistry Analysis: A representative lot detected Islet-1 and 2 homeobox in Immunocytochemistry applications (Wang, Y., et al. (2019). Nat Commun. 10(1): 4137; Carvelli, A., et al. (2022). EMBO J. 41(13): e108918).
Western Blotting Analysis: A representative lot detected Islet-1 and 2 homeobox in Western Blotting applications (Baleriola, J., et al. (2016). Sci Rep. 6: 25928).
Electrophoretic Mobility Shift Assay: A representative lot detected Islet-1 and 2 homeobox in Electrophoretic Mobility Shift Assay applications (Du, A., et al. (2009). Diabetes. 58(9);:2059-69).
Immunohistochemistry Applications: A representative lot detected Islet-1/2 homeobox in Immunohistochemistry applications (Chen, Y.Y., et al. (2007). J Neurosci. 27(31): 8448-56; Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Bielle, F., et al. (2011). Neuron. 69(6):1085-98; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Bornhorst, D., et al. (2019). Nat Commun. 10(1): 4113; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).
Immunofluorescence Analysis: A representative lot detected Islet-1/2 homeobox in Immunofluorescence applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in rat hypothalamus tissue lysate.
Western Blotting Analysis: A 1:1000 dilution of this antibody detected Islet-1/2 homeobox in rat hypothalamus tissue lysate.
Tested Applications
Flow Cytometry Analysis: A representative lot detected Islet-1 and 2 homeobox in Flow Cytometry applications (Lei, I., et al. (2020). Front Cell Dev Biol. 7: 358).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Islet-1 and 2 homeobox in Chromatin Immunoprecipitation applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Ma, Q., et al. (2020). Cell Rep. 31(3): 107532).
Immunocytochemistry Analysis: A representative lot detected Islet-1 and 2 homeobox in Immunocytochemistry applications (Wang, Y., et al. (2019). Nat Commun. 10(1): 4137; Carvelli, A., et al. (2022). EMBO J. 41(13): e108918).
Western Blotting Analysis: A representative lot detected Islet-1 and 2 homeobox in Western Blotting applications (Baleriola, J., et al. (2016). Sci Rep. 6: 25928).
Electrophoretic Mobility Shift Assay: A representative lot detected Islet-1 and 2 homeobox in Electrophoretic Mobility Shift Assay applications (Du, A., et al. (2009). Diabetes. 58(9);:2059-69).
Immunohistochemistry Applications: A representative lot detected Islet-1/2 homeobox in Immunohistochemistry applications (Chen, Y.Y., et al. (2007). J Neurosci. 27(31): 8448-56; Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Bielle, F., et al. (2011). Neuron. 69(6):1085-98; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Bornhorst, D., et al. (2019). Nat Commun. 10(1): 4113; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).
Immunofluorescence Analysis: A representative lot detected Islet-1/2 homeobox in Immunofluorescence applications (Du, A., et al. (2009). Diabetes. 58(9): 2059-69; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Vieira, J.R., et al. (2022). Neuron. 110(24): 4074-4089).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Insulin gene enhancer protein ISL-1 (UniProt: P61374; also known as Islet-1) is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 is a DNA-binding transcriptional activator that is predominantly localized in nucleus and, expressed in pancreatic islets and is essential for the maturation, proliferation, and survival of the endocrine pancreas. It also plays a crucial role in the development of various tissues, including the heart, motor neurons, and sensory neurons. It recognizes and binds to the consensus octamer binding site 5′-ATAATTAA-3′ in promoter of target genes. Its DNA binding region is localized in amino acids 181-240. Islet-1 also contains two LIM Zinc-binding domains (aa 17-70 and 79-133) and a LIM-binding domain (aa 262-291). Two isoforms of islet-1 have been described that are produced by alternative splicing. It plays a key role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. It is reported to be essential for cardiac development and is considered as a marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells. It also controls the expression of fibroblast growth factor and bone morphogenetic protein. Islet-1 expression is a reliable marker for pancreatic endocrine tumors and their metastases. Its expression is tightly regulated, and mutations in the ISL1 gene have been associated with congenital heart defects and developmental disorders. (Ref.: Sci Rep. 6:25928; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Wang, Y., et al. (2019). Nat Commun. 10(1):4137; Bornhorst, D., et al. (2019). Nat Commun. 10(1):4113; Ma, Q., et al. (2020). Cell Rep. 31(3):107532; Lei, I., et al. (2020). Front Cell Dev Biol. 7:358; Vieira, J.R., et al. (2022). Neuron. 110(24):4074-4089; Carvelli, A., et al. (2022). EMBO J. 41(13):e108918.)
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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