Zinc finger homeobox protein 3 (UniProt: Q15911; also known as AT motif-binding factor 1, AT-binding transcription factor 1, ATBF1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3) is encoded by the ZFHX3 (also known as ATBF1) gene (Gene ID: 463) in human. ATBF1 contains 23 C2H2-type zinc fingers and 4 homeobox DNA-binding domains. It is shown to translocate from the cytoplasm to the nucleus following TGF-beta stimulation. It acts as a transcriptional regulator that can act as an activator or a repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling, it can repress the transcription of AFP via its interaction with SMAD2/3. ATBF1 is shown to regulate myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. It is not found in normal gastric mucosa, but is found in gastric carcinoma cells and its expression is higher in ER-positive breast tumors than ER-negative breast tumors. It is shown to down-regulate the MUC5AC promoter in gastric cancer cells.
Specificity
Clone 5A1.1 recognizes Zinc finger homeobox protein 3 (ATBF1) in human cells. It targets an epitope with in 87 amino acids from the C-terminal region. This antibody recognizes isoform of 306 and 404 kDa.
Immunogen
GST/His-tagged recombinant fragment corresponding to 87 amino acids from the C-terminal region of human Zinc finger homeobox protein 3.
Application
Anti-ATBF1, clone 5A1.1, Cat. No. MABN1559, is a highly specific mouse monoclonal antibody that targets Zinc finger homeobox protein 3 and has been tested in Western Blotting.
Quality
Evaluated by Western Blotting in SHSY-5Y cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected ATBF1 in 10 µg of SHSY-5Y cell lysate.
Target description
~404 kDa observed; 404.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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