MABF574

Anti-CD4 Antibody (human), PerCP-Cy5.5, clone OKT4

clone OKT4, from mouse

• Synonym(s): T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4
• UNSPSC Code: 12352203
• NACRES: NA.44
• eCl@ss: 32160702
• Conjugate: PerCP-Cy5.5
• Clone: OKT4, monoclonal
• Application: FACS, ICC, IF, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: PerCP-Cy5.5
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: OKT4, monoclonal
• species reactivity: human
• packaging: pkg of 5 μL (0.25 μg/test)
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG2bκ
• UniProt accession no.: P01730
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD4(920)

Description

General description

The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression. It is cross-reactive with CD4 in several non-human species, including Chimpanzee, Cynomolgus and Rhesus. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065)

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This mouse monoclonal antibody Anti-CD4 (human), PerCP-Cy5.5, clone OKT4 validated for use in western blotting, IP, ICC, Immunofluorescence & Flow Cytometry for the detection of CD4.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, <0.2% BSA, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD4 in one million human peripheral blood lymphocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable