MABF550

Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5

clone PC61.5, 0.2 mg/mL, from rat

• Synonym(s): Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25
• UNSPSC Code: 12352203
• NACRES: NA.44
• eCl@ss: 32160702
• Conjugate: PerCP-Cy5.5
• Clone: PC61.5, monoclonal
• Application: FACS, IHC, IP
• Citations: -

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: PerCP-Cy5.5
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PC61.5, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1λ
• UniProt accession no.: P01590
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Il2Ra(16184)

Description

General description

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.
The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.

Application

This rat monoclonal antibody Anti-CD25 (mouse), PerCP-Cy5.5, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: nwg