MABE901

Anti-TAF15 Antibody, clone 4D71

clone 4D71, from mouse

• Synonym(s): TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 4D71, monoclonal
• Application: ICC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4D71, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_003478
• UniProt accession no.: Q92804
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TAF15(8148)

Description

General description

TAF15, also known as TATA-binding protein-associated factor 2N, or 68 kDa TATA-binding protein-associated factor, or TAF(II)68, TAFII68, or RNA-binding protein 56, and encoded by the gene TAF15/RBP56/TAF2N, is a RNA and ssDNA binding protein important in transcription initiation. TAF15 aligns with the RNA polymerase II (Pol II) multiprotein complex that together with other proteins like TBP initiates transcription. TAF15 is ubiquitously expressed, and TAF15 shuttles from the nucleus to the cytoplasm upon activation; methylation of TAF15 appears to guide nuclear localization and transcriptional activity. Translocations involving the TAF15 gene may play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene are associated with amyotrophic lateral sclerosis (ALS) diseases.

~68 kDa observed

Immunogen

Recombinant protein corresponding to human TAF15.

Application

Anti-TAF15 Antibody, clone 4D71 is an antibody against TAF15 for use in western blotting & ICC.

Immunocytochemistry Analysis: A 1:500 to a 1:1,000 dilution from a representative lot from an independent laboratory detected TAF15 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 μg/mL of this antibody detected TAF15 in 10 μg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 12 - Non Combustible Liquids