MABE670

Anti-Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2

clone 5E2.2, from mouse

• Synonym(s): H3K27Ac, Histone H3 (acetyl K27), H3 histone family member T, histone cluster 3, H3t
• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Clone: 5E2.2, monoclonal
• Species reactivity: human
• Application: DB, ICC, WB
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5E2.2, monoclonal
• species reactivity: human
• technique(s): dot blot: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_009564
• UniProt accession no.: P61830
• shipped in: ambient
• target post-translational modification: acetylation (Lys27)
• Gene Information: human ... H3C1(8350)

Description

General description

Histone H3.1t (UniProt: Q16695; also known as H3K27Ac, Histone H3 (acetyl K27), H3 histone family member T, histone cluster 3, H3t) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone acetylation and deacetylation are reversible and play a major role in determining chromatin structure and function. Histone acetylation occurs mainly on the amino-terminal tail domains of histones. Acetylation neutralizes positive charges on histone and promotes the less condensed and more accessible chromatin conformation known as euchromatin. Histone acetylation is vital in regulating transcriptional activation and DNA replication, recombination, and repair. Histone H3 is one of the five ain histone proteins involved in the structure of chromatin in eukaryotic cells. It is the most extensively modified of the five histones. It features a main globular domain and a long N-terminal tail. Histone H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

~17 kDa observed; 15.5 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

KLH-cojugated peptide corresponding to a sequence around lysine 27 of yeast histone H3.

Application

Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2, Cat. No. MABE670, is a highly specific mouse monoclonal antibody, that targets Acetyl Histone H3 (Lys27) and has been tested in Western Blotting and Immunocytochemistry and Dot Blot.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in Hela cells.



Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Research Category
Epigenetics & Nuclear Function

Biochem/physiol Actions

Clone 5E2.2 detects histone H3 acetylated on lysine27 in human.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HeLa cells stimulated with Sodium Butyrate.


Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl Histone H3 (Lys27) in 10 µg of HeLa cells stimulated with Sodium Butyrate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: 05-1334

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable