MABE647

Anti-acetyl-Histone H3 (Lys27) Antibody, clone RM172

clone RM172, from rabbit

• Synonym(s): Histone H3.3, Histone H3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: RM172, monoclonal
• Species reactivity: human
• Application: WB, multiplexing
• Citations: 15

Properties

• Quality Segment: 100
• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RM172, monoclonal
• species reactivity: human
• technique(s): multiplexing: suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P84243
• shipped in: wet ice
• target post-translational modification: acetylation (Lys27)
• Gene Information: human ... H3F3B(3021)

Description

General description

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunogen

A linear peptide corresponding to human Histone H3 acetylated at Lys27.

Application

Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This Anti-acetyl-Histone H3 (Lys27) Antibody, clone RM172 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.

Biochem/physiol Actions

This antibody reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in Histone H3.

Physical form

Format: Purified

Protein A purified

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.

Western Blotting Analysis (WB): 1 μg/mL of this antibody detected acetyl-Histone H3 (Lys27) in sodium butyrate treated HeLa acid extract.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash