MABD89

Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84

clone mab 84, from mouse

• Synonym(s): Podocalyxin-like protein I, GCTM-2 antigen, Gp200
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: mab 84, monoclonal
• Application: FACS, ICC, WB
• Citations: 2

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: mab 84, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_001018121
• UniProt accession no.: O00592
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PODXL(5420)

Description

General description

55 kDa calculated

Podocalyxin-like protein 1 (PODXL) is a transmembrane protein known to be heavily sialyated and sulfated that is localized on the apical surface of podocytes and on vascular endothelia. Podocalyxin protein is expressed on the surface of undifferentiated human embryonic stem cells. Originally, the protein was characterized as a marker for vasculature and podocytes, but expression has since been observed in platelets, hematopoietic precursor cells and mesothelial cells from the coelmic cavity lining. Studies have outlined two distinct and seemingly adverse roles for this protein; it acts as an anti-adhesion molecule in renal glomerulae podocytes, but functions as an adhesion molecule within vasculature on high endothelial venules. Podocalyxin expression has been implicated in the development of aggressive cancers, beta catenin signaling, angiogenisis, and both myeloid and acute lymphoblastic leukemias.

Immunogen

HES-3 Human embryonic stem cell line

Application

Anti-Podocalyxin-like protein I (Cytotoxic) Antibody, clone mAb 84 is an antibody against Podocalyxin-like protein I (Cytotoxic) for use in Western Blotting, ICC, Flow Cytometry.

This antibody was shown to induce cell death of undifferentiated, but not differentiated hESC, within 30 minutes of incubation (Choo, A., et al. (2008). Stem Cells 26:1454-1463). This antibody has been shown to work in Western Blot applications (Choo, A., et al. (2008). Stem Cells, 26:1454–1463).
This antibody has been shown to work in Flow Cytometry applications (Choo, AB., et al. (2008). Stem Cells, 26:1454–1463).

Biochem/physiol Actions

This antibody recognizes the extracellular domain of Podocalyxin-like protein 1.
This antibody has been shown to select against undifferentiated human embryonic stem cells (hESC) and to be functionally cytotoxic against these undifferentiated hESC populations (Choo, A., et al., 2008).

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Podocalyxin-like protein I in H9 human embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB4414

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2