MABD24

Anti-NANOG Antibody, clone 7F7.1

clone 7F7.1, from mouse

• Synonym(s): Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7F7.1, monoclonal
• Application: ICC, WB
• Citations: 40

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7F7.1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_079141
• UniProt accession no.: Q9H9S0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NANOG(79923)

Description

General description

NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.

~39 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human NANOG.

Application

Anti-NANOG Antibody, clone 7F7.1 is an antibody against NANOG for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
H9 human embryonic stem cell lysate

Evaluated by Western Blot in H9 human embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable