MABC681

Anti-ETV4 Antibody, clone 1A2G3

ascites fluid, clone 1A2G3, from mouse

• Synonym(s): ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1A2G3, monoclonal
• Application: WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1A2G3, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1
• UniProt accession no.: P43268
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ETV4(2118)

Description

General description

ETS translocation variant 4 protein, alternatively known as Adenovirus E1A enhancer-binding protein E1A-F, or Polyomavirus enhancer activator 3 homolog (PEA3), is encoded by the human gene named ETVA/E1AF/PEA3. ETV4 is a protein that is a transcriptional activator that was first recognized as such because it allowed for the expression of the adenovirus E1A gene upon infection. Today ETV4 has been shown to be active in a wide variety of biological processes from stem cell differentiation, to mammary gland and duct morphogenesis to motor neuron and axon guidance. ETV4 activity is regulated via the ERK/MAP signaling and a complex interplay of post-translation modifications such as sumoylation and ubiquitination. In cancers, ETV4 activates HIF and its subsequent proliferation genes and overexpression in prostate cells leads to oncogenesis. EMD-Millipore’s Anti-ETV4 mouse monoclonal antibody has been tested in western blot against K562 cell lysates as well as recombinantly expressed ETV4 protein.

~54 kDa observed. Uncharacterized bands may appear in some lysate(s).

Immunogen

Purified recombinant fragment of human ETV4 expressed in E. Coli.

Application

Detect ETS translocation variant 4 protein using this mouse monoclonal antibody, Anti-ETV4 Antibody & clone 1A2G3 validated for use in western blotting.

Analysis Note

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ETV4 in K562 cell lysate.

Optimal working dilutions must be determined by end user.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable