MABC588

Anti-MgcRacGAP/CYK4 Antibody, clone 5G5

clone 5G5, from mouse

• Synonym(s): Rac GTPase-activating protein 1, Male germ cell RacGap, MgcRacGAP, Protein CYK4 homolog, CYK4, HsCYK-4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 5G5, monoclonal
• Application: ICC, IP, WB
• Citations: -

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5G5, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001119575

Description

General description

MgcRacGAP/CYK4 is a cytoskeletal interacting protein that participates in cellular functions from cell division and differentiation to ion transport, spermatogenesis and protein transport. MgcRacGAP/CYK4 is a component of the multi-protein complex called the centralspindlin complex that is key to cytokinesis function. MgcRacGAP/CYK4 developmentally is key for embryogenesis, adipocyte and myoblast development. It is also key in male germ development. MgcRacGAP/CYK4 associates with various proteins including alpha, beta and gamma tubulin, RACGAP1, and KIF23. MgcRacGAP/CYK4 can be localized in the nucleus but also in the cytoplasm where it is associated with the cytoskeletal spindle and microtubules. MgcRacGAP/CYK4 is highly expressed in testis, thymus and placenta but also expressed in spleen and various lymphocytes and sperm.

~75 kDa observed

Immunogen

Linear peptide corresponding to Human MgcRacGAP/CYK4.

Application

Immunocytochemistry Analysis: A representative lot of this antibody detected MgcRacGAP/CYK4 in HeLa cells (Uehara, R., et al. (2013) JCB. 202(4):623-636).

Immunoprecipitation Analysis: A representative lot of this antibody detected MgcRacGAP/CYK4 by immunoprecipitation.

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

This Anti-MgcRacGAP/CYK4 Antibody, clone 5G5 is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of MgcRacGAP/CYK4.

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2bκ in buffer containing PBS without preservatives with 50% glycerol.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Evaluated by Western Blotting in HeLa nuclear extract cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MgcRacGAP/CYK4 in 10 µg of HeLa nuclear extract cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 12 - Non Combustible Liquids