MABC582

Anti-hIAP-1/BIRC3 Antibody, clone 11D12.1

clone 11D12.1, from mouse

• Synonym(s): Baculoviral IAP repeat-containing protein 3, Apoptosis inhibitor 2, API2, C-IAP2, IAP homolog C, Inhibitor of apoptosis protein 1, IAP-1, hIAP-1, hIAP1, RING finger protein 49, TNFR2-TRAF-signaling complex protein 1, hIAP-1/BIRC3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 11D12.1, monoclonal
• Application: WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11D12.1, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001156
• UniProt accession no.: Q13489
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... BIRC3(330)

Description

General description

Baculoviral IAP repeat-containing protein 3 (UniProt Q13489; also known as API2, Apoptosis inhibitor 2, Baculoviral IAP repeat-containing 3, c-IAP2, hIAP-1, hIAP1, IAP homolog C, IAP-1, Inhibitor of apoptosis protein 1, Mammalian IAP homolog C, RING finger protein 49, TNFR2-TRAF-signaling complex protein 1) is encoded by the BIRC3 (also known as API2, AIP1, IAP1, HAIP1, HIAP1, MALT2, MIHC, RNF49) gene (Gene ID 330) in human. hIAP-1 (c-IAP2) belongs to the inhibitor of apoptosis (IAP) family of proteins characterized by the presence of at least one baculoviral IAP repeat (BIR) domain. hIAP-1 (c-IAP2 contains three BIR (BIR1/2/3) domains, followed by an UBA (ubiquitin-associated), a CARD (caspase activation and recruitment domain), and a RING (really interesting new gene) domain. The BIR domains mediate hIAP-1 interaction with various binding partners, such as caspases, other IAP family members, and receptor-associated complexes. The RING domain and its associated E3 ligase mediates not only the ubiqutination of substrate proteins, such as caspase-3, caspase-7, SMAC/DIABLO, but also self-ubiquitylation. The UBA domain enables hIAP-1 to engage protein complexes containing poly-ubiquitylated protein(s).

~60 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to the BIR 1/2 of human hIAP-1/BIRC3.

Application

Detect BIRC3 using this mouse monoclonal Anti-hIAP-1/BIRC3 Antibody, clone 11D12.1, Cat. No. MABC582, validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected hIAP-1/BIRC3 in 10 µg of TF-1, Jurkat, and Molt-4 cell lysate.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected hIAP-1/BIRC3 in 10 µg of K562 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1