MABC43
Anti-Mcl-1 Antibody, clone RC13
clone RC13, from mouse
Synonym(s):
myeloid cell leukemia sequence 1 (BCL2-related), myeloid cell leukemia sequence 1, isoform 1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, myeloid cell leukemia ES, induced myeloid leukemia cell differentiation protein Mcl-1
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RC13, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... MCL1(4170)
General description
Mcl-1 (Induced myeloid leukemia cell differentiation protein) is a potential transmembrane protein associated with the mitochondria belonging to the Bcl-2 family of proteins. Members of the Bcl-2 family have known participation in regulatory roles for apoptosis, cell growth, and differentiation. The hydrophobic carboxyl terminus is conserved among members of this family and in other members has proven to be a transmembrane region. Mcl-1 is found to be expressed early in myeloid differentiation. Two separate isoforms of Mcl-1 exist with opposing functions. Full length Mcl-1 (Isoform 1) promotes cell viability, over proliferation. Isoform 2 results from the skipping of the central exon of Mcl-1 during mRNA processing and the joining of the first and third exons. The function of the resulting protein induces apoptosis when accumulated. Pro-apoptotic function is shown to increase when isoform 1 and 2 dimerize.
Immunogen
Recombinant protein corresponding to human Mcl-1.
Application
Detect Mcl-1 using this Anti-Mcl-1 Antibody, clone RC13 validated for use in WB, IH & IP.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in follicular lymphoma, diffuse large cell lymphoma, and polymorphic lymphoma tissues. (Zhou, P., et al. (2001). Blood. 97(12):3902-3909).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Han, J., et al. (2005). Biological Chemistry. 280:16383-16392).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Han, J., et al. (2005). Biological Chemistry. 280:16383-16392).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Quality
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Mcl-1 in 10 µg of Raji cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Mcl-1 in 10 µg of Raji cell lysate.
Target description
~37 kDa observed
Linkage
Replaces: MAB4602
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Raji cell lysate
Raji cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Qi Shen et al.
Cell death & disease, 9(10), 986-986 (2018-09-27)
Lung cancer is the leading cause of death in the United States, with non-small cell lung cancers (NSCLC) accounting for 85% of all cases. By analyzing the expression profile of the pro-apoptotic and anti-apoptotic proteins, we have assigned NSCLCs into
Lindy Vernooij et al.
Molecular cancer therapeutics, 20(6), 1161-1172 (2021-04-15)
Neuroblastoma tumors frequently overexpress the anti-apoptotic protein B-cell lymphoma/leukemia 2 (BCL-2). We previously showed that treating BCL-2-dependent neuroblastoma cells with the BCL-2 inhibitor venetoclax results in apoptosis, but unfortunately partial therapy resistance is observed. The current study describes the identification
Raju R Rayavarapu et al.
The Journal of biological chemistry, 290(14), 8722-8733 (2015-02-15)
The metastasis of cancer cells from the site of the primary tumor to distant sites in the body represents the most deadly manifestation of cancer. In order for metastasis to occur, cancer cells need to evade anoikis, which is defined
Adriana E Tron et al.
Nature communications, 9(1), 5341-5341 (2018-12-19)
Mcl-1 is a member of the Bcl-2 family of proteins that promotes cell survival by preventing induction of apoptosis in many cancers. High expression of Mcl-1 causes tumorigenesis and resistance to anticancer therapies highlighting the potential of Mcl-1 inhibitors as
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service