MABC196

Anti-Chitinase-3-like protein 1 (YKL-40) Antibody, clone mAY

clone mAY, from mouse

• Synonym(s): Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, CGP-39, GP-39, hCGP-39, YKL-40
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: mAY, monoclonal
• Application: IHC, WB, activity assay
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: mAY, monoclonal
• species reactivity: human
• technique(s): activity assay: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001267
• UniProt accession no.: P36222
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CHI3L1(1116)

Description

General description

YKL-40 (Chitinase-3-like protein 1; human cartilage glycoprotein 39) is a secreted glycoprotein belonging to the glycosyl hydrolase 18 (GH18) family. It is found primarily in chondrocytes, activated macrophages, and hepatocytes. The precise biological function of YKL-40 is still unknown; however, recent studies suggest that YKL-40 may play a role in cell differentiation and inflammation. YKL-40 is elevated in the sera of patients with inflammatory diseases or cancer, and it is currently being investigated as a potential biomarker for these conditions.

~42 kDa observed

Immunogen

Recombinant protein corresponding to human Chitinase-3-like protein 1 (YKL-40).

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Chitinase-3-like protein 1 (YKL-40) in U87 cells (This protein blocks tumor angiogenesis tube formation and progression (Faibish, M., et al. (2011). Mol Cancer Ther. 10(5):742-751.)).

Activity Assay Analysis: A representative lot from an independent laboratory neutralized Chitinase-3-like protein 1 in a neutralization assay (Faibish, M., et al. (2011). Mol Cancer Ther. 10(5):742-751.).

Research Category
Cell Structure

Research Sub Category
Developmental Signaling

This Anti-Chitinase-3-like protein 1 (YKL-40) Antibody, clone mAY is validated for use in Western Blotting, IHC, Activity Assay for the detection of Chitinase-3-like protein 1 (YKL-40).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
MG-63 conditioned media

Evaluated by Western Blot in MG-63 conditioned media.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Chitinase-3-like protein 1 (YKL-40) in 10 µg of MG-63 conditioned media (This protein blocks tumor angiogenesis tube formation and progression (Faibish, M., et al. (2011). Mol Cancer Ther. 10(5):742-751.)).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2