MAB995

Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3

ascites fluid, clone 3F6B3, Chemicon^®

• Synonym(s): PG
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 3F6B3, monoclonal
• Species reactivity: bacteria
• Application: DB, ELISA, ICC
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 300
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 3F6B3, monoclonal
• species reactivity: bacteria
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable
• isotype: IgG1
• shipped in: wet ice

Description

Immunogen

Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.

Application

May be used in solid-phase or competitive ELISA, immunodot-blot and immunocytochemistry formats. Although PG is a bacterial surface component, pre-treatments, such as treatment with strong acid (25mM HCl), for Gram-positive bacteria, or with a detergent, such as SDS or Triton-X (1% in PBS), for Gram-negative bacteria, may be necessary to expose the epitopes.

Optimal working dilutions must be determined by end user.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

This Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3 is validated for use in DB, ELISA, IC for the detection of Bacterial Peptidoglycan.

Biochem/physiol Actions

Specific for the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues, and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).

Physical form

Ascites fluid, no preservatives. Liquid.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable