MAB80138 anti hMPV antibody was used in Immunofluorescence in the following study. A549 or WI-38 cells were fixed in PBS containing 4% paraformaldehyde for 10 minutes on ice, followed by permeabilization with 0.1% saponin for 30 minutes and blocking with PBS containing 10% fetal bovine serum and 2.5% bovine serum albumin for 60 minutes at room temperature. The primary antibody against hMPV nucleoprotein (N protein) was incubated overnight at 4°C, followed by secondary antibody GAM 647 for 30 minutes at room temperature. Nuclear staining was achieved with DAPI staining for 10 minutes. The study can be accessed at https://pmc.ncbi.nlm.nih.gov/articles/PMC5772477/.
This product is usually provided at a concentration of 1 mg/mL, and the lot-specific Certificates of Analysis can be found on the website: https://www.sigmaaldrich.com/product/Anti-Human-Metapneumovirus-Antibody-clone-507,MM_NF-MAB80138#anchor_COA
MAB80138
Anti-Human Metapneumovirus Antibody, clone 507
clone 507, Chemicon®, from mouse
Synonym(s):
hMPV
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100 μL
CZK 12,700.00
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100 μL
CZK 12,700.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
507, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG2a
shipped in
wet ice
General description
Human metapneumovirus (hMPV) is a member of the pneumovirinae subfamily of paramyxoviruses, first described in 2001 from pediatric respiratory specimens in the Netherlands. It is an enveloped pleomorphic virus with a single negative-strand RNA genome. Two major serotypes, A and B, have been described. Several studies identified hMPV in specimens worldwide, and estimated that by age 5 years 70% of children have developed antibodies to hMPV.
Specificity
Recognizes the nuclear protein. Reactive with all hMPV sub-types; A1, A2, B1, B2.
Application
Detect Human Metapneumovirus using this Anti-Human Metapneumovirus Antibody, clone 507 validated for use in ELISA, FC, IF & WB.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Estimates of individuals exposed to human metapneumovirus in a community-based Taiwanese population in 1999.
Chung-Guei Huang,Kuo-Chien Tsao,Tzou-Yien Lin,Yhu-Chering Huang,Li-Ang Lee,Tzu-Hou Chen et al.
Archives of Virology null
Simon Loevenich et al.
Mediators of inflammation, 2019, 4964239-4964239 (2019-11-07)
Human metapneumovirus (HMPV) may cause severe respiratory disease. The early innate immune response to viruses like HMPV is characterized by induction of antiviral interferons (IFNs) and proinflammatory immune mediators that are essential in shaping adaptive immune responses. Although innate immune
Xiaoyan Li et al.
Intervirology, 58(6), 403-412 (2016-04-21)
No licensed vaccines or therapeutic agents for human metapneumovirus (hMPV) infection exist to date. We aimed to construct a multi-epitope peptide (MEP) of hMPV to show promising results for epitope-based vaccine development. Six independent algorithms were screened to predict B-cell
B G van den Hoogen et al.
Nature medicine, 7(6), 719-724 (2001-06-01)
From 28 young children in the Netherlands, we isolated a paramyxovirus that was identified as a tentative new member of the Metapneumovirus genus based on virological data, sequence homology and gene constellation. Previously, avian pneumovirus was the sole member of
Youxian Li et al.
Scientific reports, 8(1), 883-883 (2018-01-19)
Thymic stromal lymphopoietin (TSLP) is associated with several allergic diseases including asthma. Two isoforms of TSLP exist in humans, a long form (lfTSLP) and a short form (sfTSLP), displaying distinct immunological functions. Recently, TSLP was found to be upregulated in
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Regarding MAB80138, anti hMPV antibody, is there any paper that was published using this antibody for Immunofluorescence? In addition was is the approximate concentration of the antibody that you will send?
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