MAB5414

Anti-Notch 1 Antibody, extracellular, clone 8G10

culture supernatant, clone 8G10, Chemicon^®

• Synonym(s): Motch A, mT14, p300
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 8G10, monoclonal
• Application: FACS, IHC, WB
• Citations: 6

Properties

• biological source: hamster (Syrian)
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 8G10, monoclonal
• species reactivity: mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_017617.3
• UniProt accession no.: P46531
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NOTCH1(4851)

Description

General description

Notch 1 is a type 1 membrane protein that serves as a receptor for the membrane bound ligands jagged-1 and -2 and delta-1 to regulate cell fate determination through the implementation of differentiation, proliferation and apoptotic programs. Upon ligand binding, the Notch intracellular domain (NICD) is released by proteolytic processing and subsequently translocates to the nucleus where it forms a transcriptionally active complex with RBP-J kappa to activate genes of the enhancer of split locus. Notch-1 may also be involved in cell specification and/or differentiation and body segment determination in the postimplantation embryo. In the adult animal, Notch-1 is highly expressed in the brain, lung, and thymus with lower expression levels in the spleen, bone-marrow, spinal cord, eyes, mammary gland, liver, intestine, skeletal muscle, kidney and heart.

Immunogen

A GST fusion protein comprising amino acids 1299-1492 of mouse Notch 1

Epitope: extracellular

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-Notch 1 Antibody, extracellular, clone 8G10 (Syrian Hamster Monoclonal Antibody) validated in FC, WB, IHC to detect Notch 1 also known as Motch A, mT14, p300.

Western blot

Immunohistochemistry on paraformaldehyde fixed frozen tissue sections.

Flow Cytometry

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with natural and recombinant Notch1. Recognizes a band migrating at approximately 300 kDa on Western blots of rat and mouse protein extracts. A cross reactive band migrating at approximately 230 kDa is observed in some samples and is believed to correspond to the processed extracellular fragment of Notch 1.

Physical form

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2 micron membrane prior to vialing. Product contains 20% FBS and Ciprofloxacin at final concentration of 10 μg/mL.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: brain, lung, thymus.

Other Notes

Replaces: 04-1046

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids