MAB3501

Anti-Caspase 2 Antibody, clone 10C6

clone 10C6, Chemicon^®, from rat

• Synonym(s): ICH-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 10C6, monoclonal
• Application: ELISA, FACS, IP, WB
• Citations: 9

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 10C6, monoclonal
• species reactivity: human, monkey, canine, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_032982.2, NM_032983.2
• UniProt accession no.: P42575
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP2(835)

Description

Immunogen

Raised against the P19 subunit of recombinant human Caspase-2 protein.

Application

Anti-Caspase 2 Antibody, clone 10C6 detects level of Caspase 2 & has been published & validated for use in ELISA, FC, IP & WB.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

Western blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.

Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.

EIA: Recommended concentration 0.5-1 μg/ml.

Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

MAB3501 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).

Physical form

Format: Purified

Purified rat monoclonal antibody in buffer containing PBS, 0.1% preservative.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-571

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable