MAB2014

Anti-OB-Cadherin Antibody, extracellular domain, clone 16A

clone 16A, Chemicon^®, from mouse

• Synonym(s): Cadherin-11
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 16A, monoclonal
• Application: ICC, IHC, WB
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: 16A, monoclonal
• species reactivity: rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001797.2
• UniProt accession no.: P55287
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CDH11(1009)

Description

General description

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

Immunogen

Epitope: extracellular domain

Fusion protein corresponding to extracellular domain of human OB-cadherin coupled to GST.

Application

Detect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blot: 1:25 - 1:250
Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol.
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells
Optimal working dilutions must be determined by the end user.I/

Biochem/physiol Actions

Reacts with the extracellular domain of human cadherin-11.

Physical form

Affinity purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide.

Format: Purified

Preparation Note

1 year at 4°C from date of shipment

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2