MAB1998

Anti-Integrin α2β1 Antibody, clone BHA2.1

clone BHA2.1, Chemicon^®, from mouse

• Synonym(s): VLA-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: BHA2.1, monoclonal
• Application: FACS, IHC (p), IP
• Citations: 48

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BHA2.1, monoclonal
• species reactivity: human, canine, pig
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NM_002203.3
• UniProt accession no.: P17301
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGA2(3673)

Description

Application

Anti-Integrin α2β1 Antibody, clone BHA2.1 is an antibody against Integrin α2β1 for use in FC, IH, IH(P) & IP.

Research Category
Cell Structure

Research Sub Category
Integrins

flow cytometry: 10 μg/mL

Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1

immunohistochemistry in frozen tissue sections: 5 μg/mL

Immunohistochemistry in paraffin sections: 1:50. Block endogenous peroxidase using 0.5% H202 in 80% ethanol, 30 min and unspecific reactions using NGS 1:5 in PBS, 20min RT. Bouin′s fixative solution has been used successfully. Incubation time, conditions: 24 h; 4°C; pretreatment: 0.1% trypsin, 30 min, 37°C. Intracytoplasmic staining near cell membranes.

Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.

Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.

Final working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with the collagen receptor VLA-2 (alpha-2beta-1) integrin complex.

Physical form

Format: Purified

Protein A purified

Purified immunoglobulin from Protein A chromatography. Presented in liquid in 0.02M PB pH 7.6, 0.25M NaCl, 0.1% sodium azide.

Preparation Note

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Skin, CD4 & CD8 positive T cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable