FCMAB282F

Milli-Mark^® Anti-Integrin αVβ3 Antibody, clone LM609-FITC

clone LM609, Milli-Mark^®, from mouse

• Synonym(s): Vitronectin Receptor, CD51/CD61, integrin alphaVbeta3, alphaVbeta3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: LM609, monoclonal
• Application: FACS
• Citations: 1

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LM609, monoclonal
• species reactivity: human
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG1
• NCBI accession no.: NP_002201
• UniProt accession no.: P06756
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGAV(3685)

Description

General description

The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.

Immunogen

Purified adhesion receptor from the human melanoma cell line M21 (Cherish, D. and Spiro, R. (1987) J. Biol. Chemistry, 262(36):17703-17711).

Application

Detect Integrin αVβ3 using this Milli-Mark Anti-Integrin αVβ3 Antibody, clone LM609-FITC validated for use in FC.

Research Category
Cell Structure

Research Sub Category
Integrins

Biochem/physiol Actions

Antibody recognizes Integrin αVβ3.

Physical form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Flow Cytometry
Antibody dilution for cellular staining:

• Prepare an antibody working solution by diluting the primary antibody 1:5 with PBS
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments

Analysis Note

Control
U937 cells

Evaluated by flow cytometry using U937 cells.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2