FCMAB176F

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25

clone DK25, Milli-Mark^®, from mouse

• Synonym(s): Anti-CD8 FITC, Clone DK25 Anti-CD8
• UNSPSC Code: 12352203
• NACRES: NA.44
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: DK25, monoclonal
• Application: FACS
• Citations: 3

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: DK25, monoclonal
• species reactivity: human
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001759.3
• UniProt accession no.: P01732
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD8A(925)

Description

General description

68 kDa (Nakuchi, 1997)

CD8 is a 68 kDa disulphide-linked, transmembrane glycoprotein. The CD8 molecule serves as a receptor for MHC class I molecules and can mediate a function as a coreceptor in TCR-ligand binding and T-cell activation. Its cytoplasmic part is associated with the p56Ick tyrosine kinase (Nakuchi, 1997). CD8 is expressed by the great majority of cortical thymocytes and approximately 30% of medullary thymocytes, and by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells. In addition a proportion of gamma delta T cells and NK cells express CD8 (Nakuchi, 1997). Thus, CD8 is expressed by about 25-35% of peripheral T cells, and about 30% of NK cells express low levels of CD8 (Leong, 1999). The specificity of Anti-CD8, DK25, is equivalent to that of the CD8-clustered antibodies OKT8 and Leu-2a (Stein, 1984).

Application

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25 detects level of CD8 & has been published & validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

Antibody recognizes Human CD8 antigen.

Physical form

Protein A purified

Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Preparation Note

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Human Lympnocytes.

Evaluated by flow cytomatry using human lymphocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2