F1903

Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified

• UNSPSC Code: 12352202
• NACRES: NA.75
• eCl@ss: 32160405
• Biological source: human
• Form: lyophilized
• Technique(s): cell culture | mammalian: suitable
• Concentration: 1 mg/mL
• Mol wt: 40 kDa

Properties

• biological source: human
• Quality Segment: 100
• form: lyophilized
• mol wt: 40 kDa
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): cell culture | mammalian: suitable
• input: sample type epithelial cells; sample type: human embryonic stem cell(s); sample type mesenchymal stem cell(s); sample type hematopoietic stem cell(s); sample type pancreatic stem cell(s); sample type induced pluripotent stem cell(s); sample type neural stem cell(s)
• NCBI accession no.: NM_002026.2, NM_054034.2, NM_212474.1, NM_212475.1, NM_212476.1, NM_212478.1, NM_212482.1
• UniProt accession no.: P02751
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... FN1(2335)

Description

General description

Heparin-binding 40 kDa fragment of human plasma fibronectin purified from chymotryptic digest of human plasma fibronectin. This preparation contains the CS-1 heparin-binding region of fibronectin. Heparin-binding fragment promotes cytoskeleton organization in integrin signaling. This preparation shows one major band at 40 kDa in SDS-PAGE with no higher molecular weight species present. Smaller proteolytic fragments may be present.

Human sourced raw material used in the production of this product tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2), human T-cell lymphotropic virus (HTLV-1 and HTLV-2) and Treponema pallidum. Handle as if potentially infectious.

Product Source: Human

Application

ELISA

Affinity chromatography

Possibly for molecular mapping of fibronectin function

Cell attachment and integrin signaling assays

Optimal working dilutions must be determined by the end user.

Physical form

Purified by gelatin-Sepharose and heparin-Sepharose column chromatography. Lyophilized from sodium citrate buffered saline, 20mM, NaCl 150mM, pH 7.4. Reconstitute with distilled water.

Preparation Note

Maintain lyophilized material at -20°C for up to one year from date of receipt. After reconstitution, maintain at -20°C for up to six months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1