CBL434

Anti-c-Myc Tag Antibody

CHEMICON^®, mouse monoclonal, 3C7

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 3C7, monoclonal
• Species reactivity: mouse, human
• Application: ELISA, IP, WB
• Citations: 6

Properties

• Product Name: Anti-c-myc Antibody, clone 3C7, clone 3C7, Chemicon^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3C7, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_002467.3
• UniProt accession no.: P01106
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MYC(4609) ; mouse ... Myc(17869)

Description

Immunogen

Epitope: a.a. 171-188

Synthetic peptide: Cys-Ser-Thr-Ser-Ser-Leu-Tyr-Leu-Gln-Asp-Leu-Ser-Ala-Ala-Ala-Ser-Glu-Cys selected from the human c-myc sequence (amino acids (171-188). The peptide was conjugated to keyhole limpet haemocyanin.

Application

Detect c-myc also known as Proto-oncogene c-Myc with Anti-c-myc Antibody, clone 3C7 (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western blotting

Immunoprecipitation

Immunoassay detection of c-myc

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

In immunoblotting this antibody detects one major band at 62-64 kDa which can be blocked by pre-incubation with the immunizing peptide. In immunoprecipitation the antibody detects a 62 kDa band from cell lysates.

KNOWN SPECIES CROSS REACTIVITY: This antibody is known to cross react with mouse c-myc

Physical form

Format: Purified

The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable