CBL1360

Anti-CD117 Antibody, clone ACK2

clone ACK2, Chemicon^®, from rat

• Synonym(s): c-kit, SCF Receptor
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: ACK2, monoclonal
• Application: FACS, IHC, IP, WB
• Citations: 8

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ACK2, monoclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• input: sample type hematopoietic stem cell(s); sample type mesenchymal stem cell(s)
• isotype: IgG2bκ
• NCBI accession no.: NM_000222.2, NM_001093772.1
• UniProt accession no.: P10721
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KIT(3815)

Description

General description

145 kDa

This 145 kDa molecule is a member of the tyrosine kinase receptor family and is expressed by a majority of hematopoietic progenitor cells. The interaction of the mouse c-kit receptor and steel factor promotes proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells, and plays a role in signaling and activation of these cells.

Application

Detect CD117 using this Anti-CD117 Antibody, clone ACK2 validated for use in FC, IH, IP, FUNC & WB.

Flow Cytometry: 0.2 μg per 10e6 cells

Immunoprecipitation

Immunohistochemistry (Frozen)

Western blot

Blocking Ligand Binding

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Mesenchymal Stem Cells

Biochem/physiol Actions

Specifically recognizes mouse CD117, known as c-kit receptor, stem cell factor receptor and steel factor receptor.

Physical form

Format: Purified

Protein A purified

The monoclonal is presented in phosphate buffered saline containing 10mM sodium azide.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Analysis Note

Control
Skin tissue, normal breast epithelium, melanocytes, mast cells and glial cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: CBL1360P

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids