AG230

Glial Fibrillary Acidic Protein, porcine

• Synonym(s): GFAP
• UNSPSC Code: 12352106
• NACRES: NA.41
• eCl@ss: 32160405
• Form: liquid
• Assay: >99% (pure GFAP)
• Biological source: Porcine
• Mol wt: calculated mol wt 49.44 kDa (428 a.a. spliced isoform; NP_001231326), calculated mol wt 53.63 kDa (468 a.a. spliced isoform; XP_005668766)

Properties

• biological source: Porcine
• Quality Segment: 100
• assay: >99% (pure GFAP)
• form: liquid
• mol wt: calculated mol wt 49.44 kDa (428 a.a. spliced isoform; NP_001231326), calculated mol wt 53.63 kDa (468 a.a. spliced isoform; XP_005668766)
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): ELISA: suitable, radioimmunoassay: suitable, western blot: suitable
• NCBI accession no.: NM_001244397
• UniProt accession no.: F1RR02
• shipped in: dry ice
• storage temp.: -10 to -25°C
• Gene Information: pig ... GFAP(396562)

Description

General description

Glial fibrillary acidic protein (UniProt F1RR02) is encoded by the GFAP gene (Gene ID 396562) in porcine. GFAP is a type III intermediate filament (IF) thought to help maintain astrocyte mechanical strength and is closely related to vimentin, desmin, and peripherin, which are involved in the structure and function of cellular cytoskeleton. GFAP is expressed in astrocytes in the central nervous system (CNS), where it is involved in many important CNS processes, including cell communication and the blood brain barrier (BBC) function. GFAP contains the characteristic tripartite domain structure among IF members, a central α-helical rod domain (a.a. 73-377) flanked by a non-α-helical N-terminal head (a.a. 1-72) and a C-terminal tail (a.a. 378-432) domain. The head and tail domains are most structurally variable among IF proteins and their contributions to filament assembly vary depending on the IF proteins. For GFAP, the tail domain is reported to be important for filament assembly. The tail domain R416W mutation is linked to the neurological disorder Alexander disease (AxD).

Purified porcine Glial Fibrillary Acidic Protein (GFAP) purified from spinal cord.

Physical form

Liquid. Buffer = PBS.

Preparation Note

Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analysis Note

a 50 kDa single band by SDS-PAGE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2