AG100

Human IgG Fc Control Protein, recombinant protein

• UNSPSC Code: 12352203
• NACRES: NA.42
• eCl@ss: 32160702
• Assay: >90% (Coomassie blue staining, SDS-PAGE)
• Biological source: human

Properties

• biological source: human
• Quality Segment: 100
• assay: >90% (Coomassie blue staining, SDS-PAGE)
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• shipped in: dry ice

Description

General description

Product Source: Mammalian cells, HEK293 cell line

Recombinatant fusion proteins consisting of the extracellular domain of immunoregulatory proteins and the constant (Fc) domain of immunoglobulin G (IgG) represent a growing class of human therapeutics. Chimeric protein of Human target protein and Human IgG-Fc domain can be purified using protein A chromatography.
This product is the control protein, Human IgG-Fc domain, purified using protein A-agarose (Millipore Corp, cat. # 16-125). MW ~ 55 kDa.

Application

Research Category
All

This product should be used as the control protein in the activity test of chimeric protein of Human target protein and Human IgG-Fc domain.

Other applications of Human IgG-Fc domain.

Packaging

Active, as purified using protein A-agarose (Millipore Corp, cat. # 16-125).

Physical form

50µg of recombinant protein in 50µl of 1X PBS, 20%(v/v) glycerol. Frozen solution.

Preparation Note

Shipped on dry ice. Best stored at -80 C for up to 6 months after date of receipt. Thawed aliquots should be used immediately, as repeated freeze/thaw cycles might result in loss of activity.

Analysis Note

Western blotting by primary antibody Mouse anti-Human IgG, clone HP6017, Fc (Millipore Corp, cat.# MAB1302) and secondary antibody Goat anti-Mouse IgG, HRP conjugate (Millipore Corp, cat.# 12-349).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 2