ABT52

Anti-phospho-Peak Antibody (Tyr665)

from rabbit, purified by affinity chromatography

• Synonym(s): Pseudopodium-enriched atypical kinase 1, Sugen kinase 269, Tyrosine-protein kinase SgK269
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: DB, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): porcine (based on 100% sequence homology), rat (based on 100% sequence homology), orangutan (Sumatran; based on 100% sequence homology), mouse (based on 100% sequence homology)
• technique(s): dot blot: suitable, western blot: suitable
• NCBI accession no.: NP_079052
• UniProt accession no.: Q9H792
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr665)
• Gene Information: human ... PEAK1(79834)

Description

General description

220 kDa calculated

Pseudopodium-enriched atypical kinase 1 (Peak1; KIAA2002, or sgk269) is a ubiquitous non-receptor tyrosine kinase that belongs to the NFK3 family. It interacts with actin filaments via its N-terminal actin-targeting region. It also modulates the activity of multiple cytoskeletal regulatory proteins such as paxillin, p130Cas, Erk, and Crk; and mediates cell proliferation and migration via the Ras/Raf/Erk and Src-p130Cas-Crk-Paxillin pathways. Peak1 may play an important role in the progression of tumors.

Immunogen

Epitope: Phosphorylated Tyr665

KLH-conjugated linear peptide corresponding to human Peak phosphorylated at Tyr665.

Application

This phospho-Peak antibody is validated for use in WB & Dot Blot for the detection of the phospho-Peak protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in 293T cells transfected with wildtype GFP-Peak. (Dr. Richard Klemke, University of California-San Diego.)

Biochem/physiol Actions

This antibody recognizes Peak phosphorylated at Tyr665.

Analysis Note

Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins

Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.

Dot Blot (Specificity) Analysis: A representative lot blocked phospho-Peak (Tyr665) in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable