ABT45

Anti-Claudin-5 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 24

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, horse, human
• species reactivity (predicted by homology): equine (based on 100% sequence homology), ox (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_003268
• UniProt accession no.: O00501
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CLDN5(7122)

Description

General description

Claudin-5, a transmembrane protein deleted in velo-cardio-facial syndrome (TMVCF), seems to be involved in endothelial tight junctions. Claudin-5 has been exclusively found in the cell-cell borders of endothelial cells. Lower claudin-5 levels in sinusoidal endothelial cells could lead to their dysfunction during liver injuries. Levels of claudin-5 in tumor vessels could be a potential marker for hepatocellular carcinoma prognosis. The loss of claudin-5 function has been linked to carcinogenesis.

~17 kDa observed.
Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288).

Immunogen

Epitope: Cytoplasmic Domain

KLH-conjugated linear peptide corresponding to human Claudin-5 at the cytoplasmic domain.

Application

Anti-Claudin-5 Antibody detects level of Claudin-5 & has been published & validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:800 dilution from a representative lot detected Clausin-5 in prostate tissue.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Biochem/physiol Actions

This antibody recognizes Claudin-5 at the cytoplasmic domain.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human lung tissue lysate

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable