ABS521

Anti-RSK4 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Q9UK32, KS6A6_HUMAN, RPS6KA6, RSK4, Q9UK32, B2R854, Q6FHX2, Q8WX28, Q9H4S6, Ribosomal protein S6 kinase alpha-6, p90RSK6
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• species reactivity (predicted by homology): human (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_055311
• UniProt accession no.: Q9UK32
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RPS6KA6(27330)

Description

General description

RSK1, RSK2, RSK3, and RSK4 (ribosomal protein S6 kinase, 90 kDa, polypeptide 1,2,3, and 4; S6K-alpha 1,2,3, and 4) are Ser/Thr kinases which are activated by MAP Kinases. Ribosomal S6-kinase 4 (RSK4) is thought to be mapped in the critical region that is commonly deleted in X-linked deafness type 3, mental retardation, and choroideremia. It may be involved in normal neuronal development. RSK4 is also thought to play a role in non-growth factor signaling and may also be important in tumor suppression.

~83 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of Human RSK4.

Application

Immunoprecipitation analysis: 10 ug of Cat. No. ABS521, Anti-RSK4 was used to immunoprecipitate RSK4 in 0.5 mg PC12 or EGF treated PC12 cell lysate
Immunoprecipitation analysis: 5 ug of Cat. No. ABS521, Anti-RSK4 was used to immunoprecipitate RSK4 in 0.665mg of PC12 cell lysate. Uncharacterized bands may be detected at high antibody concentration or longer exposures

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

General Post-translation Modification

This Anti-RSK4 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of RSK4.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in rat PC12 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RSK4 in 10 µg of rat PC12 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: 04-1132

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1