ABS236

Anti-Cytochrome P450 (scc) Antibody, a.a. 509-526

from rabbit, purified by affinity chromatography

• Synonym(s): Cholesterol side-chain cleavage enzyme, mitochondrial, CYPXIA1, Cholesterol desmolase, Cytochrome P450 11A1, Cytochrome P450(scc)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 3

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_058982
• UniProt accession no.: P14137
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: rat ... Cyp11A1(29680)

Description

General description

As a member of the cytochrome P450 family, Cytochrome P450 Side Chain Cleavage Enzyme (scc) catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone during the first step of steroid biosynthesis. It is expressed in certain organs, such as ovaries, adrenal glands, and testis. Deficiency in CYP11A1 is commonly associated with adrenal insufficiency, and with variable degrees of disorder of sex development (DSD).

~52 kDa observed. On higher percentage gels and longer exposures, 8 kDa cleavage product can be detected.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of rat Cytochrome P450 (scc), a.a. 509-526.

Application

Immunohistochemistry Analysis: A 1:4,000-8,000 dilution from a representative lot detected Cytochrome P450 (scc) in rat adrenal gland and rat testis tissue.

This Cytochrome P450 (scc) antibody is validated for use in WB & IHC for the detection of the Cytochrome P450 (scc) protein.

Biochem/physiol Actions

This antibody recognizes the C-terminus of Cytochrome P450 (scc), a.a. 509-526.

Analysis Note

Control
Rat adrenal tissue lysate

Evaluated by Western Blot in rat adrenal tissue lysate.

Western Blot Analysis: 0.02 µg/mL of this antibody detected Cytochrome P450 (scc) in 10 µg of rat adrenal tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB1294

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable