ABN91

Anti-NeuN Antibody

from chicken, purified by affinity chromatography

• Synonym(s): RNA binding protein fox-1 homolog 3, Fox-1 homolog C
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: mouse, rat
• Application: ICC, IHC, WB
• Citations: 138

Properties

• biological source: chicken
• Quality Segment: 100, 200
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgY
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Rbfox3(52897) ; rat ... Rbfox3(287847)

Description

General description

NeuN (RNA binding protein fox-1 homolog 3; Fox-1 homolog C) is a RNA-binding protein found exclusively in the nuclei of neuronal cells. It is a member of the evolutionarily conserved Fox-1 family and is mainly involved in splicing of RNA.

~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortex cells.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in mouse brain, mouse hippocampus, and mouse frontal cortex tissues.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-NeuN Antibody is validated for use in IHC(P), Western Blotting, ICC for the detection of NeuN.

Biochem/physiol Actions

Other homologies: Human (94% sequence homology).

This antibody recognizes the N-terminus of NeuN.

Physical form

Affinity purified

Purified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain E16 tissue lysate

Evaluated by Western Blot in mouse brain E16 tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain E16 tissue lysate.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable