ABN76

Anti-Neurofilament H (200 kDa) Antibody

rabbit polyclonal

• Synonym(s): Neurofilament heavy polypeptide, Neurofilament H
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: mouse, human, rat, bovine
• Application: IHC, WB
• Citations: 3

Properties

• Product Name: Anti-Neurofilament H (200 kDa) Antibody, from rabbit, purified by affinity chromatography
• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human, rat, bovine
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_066554.2
• UniProt accession no.: P12036
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Nefh(528842) ; human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Description

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas, carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung. For moreinformation, see the Nervous System Cell Type Specific Marker chart on the Millipore website.

~200 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to bovine Neurofilament H.

Application

Immunohistochemistry Analysis: A 1 µg/mL dilution from a representative lot detected Neurofilament H in normal rat cerebellum tissue.

This Anti-Neurofilament H (200 kDa) Antibody is validated for use in WB, IH(P) for the detection of Neurofilament H (200 kDa).

Analysis Note

Control
Bovine cerebellum tissue lysate

Evaluated by Western Blot in bovine cerebellum tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurofilament H in bovine cerebellum tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB1982

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable