ABN352

Anti-NENF Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): NENF, Neuron derived neurotrophic factor, neudesin, CIR2, SPUF, SCIRP10
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• technique(s): immunohistochemistry: suitable, western blot: suitable
• UniProt accession no.: Q9UMX5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NENF(29937)

Description

General description

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.

~37 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human NENF.

Application

Detect the NENF protein using this Anti-NENF validated for use in WB & IHC.

Immunohistochemistry Analysis: 10 µg/mL of this antibody from a representative lot detected NENF in rat kidney tissue.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human kidney tissue lysate

Evaluated by Western Blot in human kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NENF in 15 µg of human kidney tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1