P53 apoptosis effector related to PMP-22 (PERP), also known as Keratinocyte-associated protein 1 (KCP-1, Krtcap1), is a member of the TMEM47 family and a component of the intercellular desmosome junctions. PERP has been shown to play an important role in the stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Recent evidence implies that PERP is a mediator of p53 induced apoptosis. P53 binds to the promoter of PERP and activates the PERP gene and consequently the translated PERP protein mediates the p53 induced apoptosis. PERP is expressed in apoptotic cells and in the stratified squamous skin epithelium of the skin and the tongue, but not in simple epithelia.
Immunogen
KLH-conjugated linear peptide corresponding to human PERP.
Application
Detect P53 apoptosis effector related to PMP-22 using this rabbit polyclonal antibody, Anti-PERP Antibody validated for use in western blotting & ICC.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected PERP in A431 cells.
Research Category Epigenetics & Nuclear Function
Research Sub Category RNA Metabolism & Binding Proteins
Quality
Evaluated by Western Blotting/Peptide Inhibition Assay in A431 cell lysate.
Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected PERP in 15 µg of A431 cell lysate.
Target description
~21 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control A431 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
