AB3202

Anti-LIM-3 Antibody

Chemicon^®, from rabbit

• Synonym(s): Anti-HMFN1661
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IHC (p), IP, WB
• Citations: 7

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: fish, frog, mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_014945.2
• UniProt accession no.: O94929
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ABLIM3(22885)

Description

Immunogen

C-terminal portion of mouse LIM-3 protein.

Application

Anti-LIM-3 Antibody is an antibody against LIM-3 for use in IP, WB, IC, IH, IH(P).

Immunohistochemistry: 1:250-1:1,000 on paraffin embedded sections. 1:3,000-1:6,000 on frozen sections. 1:4,000-1:8,000 on whole mounts. Recommended fixative MEMFA.

Immunocytochemistry: 1:200-1:500

Western blot: 1:2,500-1:5,000

Immunoprecipitation: 1:200

This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes LIM-3. This antibody does not appear to cross react with gsh-4 on tissue sections but may cross react in Western blot and immunoprecipitation.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• Storage Class: 12 - Non Combustible Liquids