AB2148P

Anti-Tumor Necrosis Factor-α Antibody

Chemicon^®, from rabbit

• Synonym(s): TNF-alpha
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, Neutral, WB
• Citations: 24

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, neutralization: suitable, western blot: suitable
• NCBI accession no.: NP_038721
• UniProt accession no.: P06804
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... TNF(7124)

Description

Immunogen

Highly pure (>98%) recombinant Mouse TNF-alpha.

Application

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody validated for use in ELISA, NEUT & WB.

ELISA: A concentration of at least 0.5μg/mL (100μL/well) of AB2148P is required to detect 0.2-0.4 ng/well of recombinant mTNF-alpha.

Western Blot: A concentration of 0.1-0.2 μg/mL of AB2148P is required to detect 1.5-3.0 ng/lane of mTNF-alpha, under either reducing or non-reducing conditions.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-a (0.50 ng/ml), a concentration of 0.010-0.015 μg/ml of AB2148P is required.

Optimal working dilutions must be determined by the end user

Biochem/physiol Actions

Reacts with Mouse Tumor Necrosis Factor-alpha (TNF-alpha), a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells.

Physical form

Immunoglobulin purified by affinity chromatography employing immobilized mTNF-alpha matrix. A sterile filtered antibody solution (1mg/mL) was lyophilized from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water.

Other Notes

Replaces: AB1450P

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable