AB19012

Anti-Anti-Laminin (LAMA1) antibody

CHEMICON^®, rabbit polyclonal

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human
• Application: ELISA, IHC, IP, WB
• Citations: 34

Properties

• Product Name: Anti-Laminin Antibody, Chemicon^®, from rabbit
• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_005559.2
• UniProt accession no.: P25391
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Immunogen

Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Application

Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.

Immunoblot: 1:500-1:2000

ELISA: 1:4000-1:8000

Immunohistochemistry: 1:50-1:200

Immunoprecipitation: Condition must be determined by the end user

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.

Physical form

Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.

Format: Purified

Preparation Note

The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H360FD
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable