AB1582

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, sheep polyclonal

• Synonym(s): ChAT
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: rat, guinea pig, rabbit
• Application: IHC, WB
• Citations: 17

Properties

• Product Name: Anti-Choline Acetyltransferase Antibody, serum, Chemicon^®
• biological source: sheep
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, guinea pig, rabbit
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_020549.3, NM_020984.2, NM_020985.2, NM_020986.2
• UniProt accession no.: P28329
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: rabbit ... Chat(100342226) ; rat ... Chat(290567)

Description

Immunogen

A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.

Application

Immunohistochemistry: 1:1,000-1:2,000

Immunoblotting

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582

STAINING OF GUINEA PIG ILEUM

1) Pin the ileum out flat and tight.

2) Fix overnight at 4°C in modified Zamboni′s (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).

3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.

4) Dissect off ALL circular muscle.

5) Preincubate with 10% normal donkey serum.

6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.

7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.

IMPORTANT NOTE: It is essential to remove ALL circular muscle.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.

Biochem/physiol Actions

Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.

Preparation Note

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids