8.51029

N-Biotinyl-NH-(PEG)₂-COOH . DIPEA (20 atoms)

Novabiochem^®

• Synonym(s): N-Biotinyl-NH-(PEG)₂-COOH . DIPEA (20 atoms), O-(N-Biotinyl-3-aminopropyl)-O′-(N-glutaryl-3-aminopropyl)-diethyleneglycol · DIPEA
• Empirical Formula (Hill Notation): C₂₅H₄₄N₄O₈S · xC₈H₁₉N
• CAS Number: 1205744-09-7
• Molecular Weight: 560.70
• MDL number: MFCD08458689
• UNSPSC Code: 12352106
• NACRES: NA.22

Properties

• Quality Segment: 200
• product line: Novabiochem^®
• assay: ≥95.0% (HPLC), ≥98% (TLC)
• form: powder
• reaction suitability: reaction type: Biotinylations
• manufacturer/tradename: Novabiochem^®
• mp: 131-136 °C
• application(s): peptide synthesis
• storage temp.: 2-8°C
• SMILES string: S1[C@H]([C@H]2NC(=O)N[C@H]2C1)CCCCC(=O)NCCOCCOCCOC(=O)O.N(C(C)C)(C(C)C)CC
• InChI: 1S/C17H29N3O7S.C8H19N/c21-14(18-5-6-25-7-8-26-9-10-27-17(23)24)4-2-1-3-13-15-12(11-28-13)19-16(22)20-15;1-6-9(7(2)3)8(4)5/h12-13,15H,1-11H2,(H,18,21)(H,23,24)(H2,19,20,22);7-8H,6H2,1-5H3/t12-,13-,15-;/m0./s1
• InChI key: YOALVTBRBBZXGM-HZPCBCDKSA-N

Description

General description

The use of this novel biotin derivative incorporates a hydrophilic 20 atom PEG spacer between the peptide and biotin. This not only greatly improves the solubility of the resultant peptide, but reduces steric hindrance between the peptide and biotin, leading to better avidin binding and higher biological activity. The biotin-PEG derivative can be easily incorporated using any standard coupling method as the final step in SPPS, immediately prior to cleavage of the peptide from the resin.

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(CMA2)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (50 mg in 1 ml NMP): clearly soluble

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Other Notes

Replaces: 01-63-0133

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable