71187

Insect PopCulture^® Reagent

Protein extraction directly from insect cell cultures

• Synonym(s): PopCulture reagent
• NACRES: NA.77
• UNSPSC Code: 41106511

Properties

• Quality Segment: 100
• form: liquid
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

Insect POPCULTURE^® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind<TMSYMBOL></TMSYMBOL> affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.

Features and Benefits

• No need to separate cells from culture media
• No need to clarify cell extracts prior to purification
• Higher protein yield due to target protein recovery from both medium and cells
• Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
• Compatible with protease inhibitor cocktails
• Ideal for high-throughput, expression-level screening and protein purification
• Compatible with transfected and baculovirus-infected insect cell cultures

Other Notes

Loomis, K., et al. 2002. inNovations15, 16.

Legal Information

This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

POPCULTURE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable