69670-M

His•Bind^® Resin

• Synonym(s): uncharged IDA agarose beads
• NACRES: NA.56
• UNSPSC Code: 41106500

Properties

• form: liquid
• manufacturer/tradename: Novagen^®
• storage condition: do not freeze
• application(s): life science and biopharma
• storage temp.: 2-8°C

Description

General description

His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.

Application

HIS-BIND^® resin have been used: in purification of HIS TAG^® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndrome, in protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes, for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.

Biochem/physiol Actions

HIS-BIND^® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the HIS-BIND^® Buffer Kit, that binds the His-tagged sequence effectively with Ni²⁺ or Co²⁺ cations that are immobilized onto the HIS-BIND^® resin. The target protein can be easily recovered by eluting with imidazole. The HIS-BIND^® resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.

Features and Benefits

• Reusable many times
• Compatible with HIS-BIND^® Buffer Kit
• Compatible with 1 mM THP

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• flash_point_f: 86.0 °F
• flash_point_c: 30 °C