650211

Trypsin, Excision Grade, Bovine Pancreas

• Synonym(s): Trypsin, Excision Grade, Bovine Pancreas
• CAS Number: 9002-07-7
• Enzyme Commission number: 3.4.21.4 (BRENDA, IUBMB)
• UNSPSC Code: 12352202

Properties

• Assay: ≥90% (HPLC)
• Quality Level: 100
• form: solid
• specific activity: ≥80 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: white
• solubility: trifluoroacetic acid: 0.01%; HCl: 1 mM
• foreign activity: Chymotrypsin ≤0.05%
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, analysis of protein structural domains and cleavage of fusion proteins.

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, protein structural domains, and cleavage of fusion proteins.

Warning

Toxicity: Irritant (B)

Unit Definition

1 unit is the amount of enzyme that will convert 1 µmol of substrate per min at 37°C in 100 mM Tris pH 8.5

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Stock solutions are stable for up to 1 month at -20°C or for up to 1 week at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1