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About This Item
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description
Merck USA index - 14, 9571
Quality Segment
assay
≥95% (SDS-PAGE)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
pI
5.8
solubility
water: 10 mg/mL
cation traces
Fe: 0.1%
storage temp.
−20°C
General description
Native holotransferrin from human plasma. Transferrin is a major iron binding protein and is found in human plasma or serum at concentrations of 3 mg/ml. Its concentration correlates with the total iron binding capacity of serum and its level is regulated by the availability of iron. It has bacteriostatic and fungistatic activity and is essential for iron utilization by the hemin-synthesizing reticulocytes. Stored in bone marrow as Tf-bound iron.
Physical form
Lyophilized from 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.4.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add NaN₃ and refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| form lyophilized solid | form lyophilized solid | form lyophilized solid | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: 10 mg/mL | solubility water: 1% (Readily soluble), 20 mM Tris-HCl buffer, pH 7.5: soluble | solubility water: 10 mg/mL | solubility water: soluble |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| cation traces Fe: 0.1% | cation traces Fe: ≥1000 ppm | cation traces Fe: <0.01% | cation traces - |
| assay ≥95% (SDS-PAGE) | assay ≥95% (FPLC) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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