616374

TRAIL, Human, Recombinant, E. coli

• Synonym(s): Apo2L, Human, Recombinant, E. coli, TNF-Related Apoptosis-Inducing Ligand, Human, Recombinant, E. coli
• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: liquid
• Assay: ≥95% (SDS-PAGE)

Properties

• assay: ≥95% (SDS-PAGE)
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• impurities: <0.1 EU/μg endotoxin (EU/μg TRAIL)
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Recombinant, human TRAIL (amino acids 114-281) expressed in E. coli. Ligand for TRAIL receptors DR4 and DR5. Displays potent antitumor activity against selected targets and induces apoptosis in a number of transformed cell lines, but not in normal tissues.

Biochem/physiol Actions

Induces apoptosis in U937 cells at 1 μg/ml within 5 h.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 500 mM NaCl, 10 mM Na₂HPO₄, 2.7 mM KCl, 2 mM KH₂PO₄, 0.1 mM DTT, ≤10% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze

(-70°C).

Other Notes

Lin, Y., et al. 2000. Mol. Cell. Biol.20, 6638.
Mitsaides, N., et al. 2000. Cancer Res.60, 4122.
Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343.
Mariani, S.M., et al. 1997. J. Cell Biol.137, 221.
Pan, G., et al. 1997. Science276, 111.
Pan, G., et al. 1997. Science277, 815.
Sheridan, J.P., et al. 1997. Science277, 818.
Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.
Wiley, S.R., et al. 1995. Immunity3, 673.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable