574777

Anti-Synapsin I Rabbit pAb

lyophilized, Calbiochem^®

• Synonym(s): Rabbit Anti-Synapsin I, Synapsin I Detection Antibody
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: human, rat, mouse, bovine
• Application: —
• Citations: 20

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized
• does not contain: preservative
• species reactivity: human, rat, mouse, bovine
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• dilution: (ELISA (1:2500-1:10,000); Frozen Sections ; Immunoblotting (1:200); Immunocytochemistry (1:500-1:2500); Immunoprecipitation (1 µg/200 µg lysate))
• isotype: IgG
• shipped in: ambient
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Syn1(20964)

Description

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.

Recognizes the synapsin I protein in rat brain.

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Immunogen

Bovine

purified, brovine brain synapsin I

Application

ELISA (1:2500-1:10,000)

Frozen Sections (see application references)

Immunoblotting (1:200)

Immunocytochemistry (1:500-1:2500)

Immunoprecipitation (1 µg/200 µg lysate)

Physical form

Lyophilized from 5 mM ammonium bicarbonate.

Preparation Note

Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat brain

Other Notes

Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.

This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1